Methods, compositions and devices for maintaining and growing human stem and/or hematopoietics cells

US5763266A · kind A · utility

Assignee

• THE REGENTS OF THE UNIVERSITY OF MICHIGAN · US

Inventors

• Bernhard O. Palsson · Village of La Jolla, US
• Stephen G. Emerson · Ann Arbor, US
• Richard Schwartz · Ann Arbor, US

Key dates

• Filing date: Nov 7, 1994
• Grant date: Jun 9, 1998
• Expiry date: Nov 7, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2502/99
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods, compositions and devices are provided for the growth of human stem and/or hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types successfully reconstructs hematopoietic tissue ex vivo. Optionally, at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. The stromal cells and hematopoietic cells may be maintained separately, and both the adherent and non-adherent cells harvested.