Process for producing solubilized protein
US5763583A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 6, 1995 |
| Grant date | Jun 9, 1998 |
| Priority date | — |
| Expiry date | Oct 6, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/4741
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the production of a solubilized protein is disclosed, which is obtained by reducing disulfide bonds in a disulfide bond-containing water-insoluble protein material into mercapto groups and subsequently converting a part or an entire portion thereof into carboxymethyldisulfide groups. In particular, the disclosed process for the production of a solubilized protein which comprises (a) treating a disulfide bond-containing water-insoluble protein material with an aqueous alkaline solution of a reducing agent, and (b) reacting the protein treated by step (a) with thioglycolic acid in the presence of an oxidizing agent under a weakly acidic to a weakly alkaline condition. A process for the production of regenerated protein products, which comprises regenerating disulfide bonds in the solubilized protein, is also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.