Disposable reagent storage and delivery cartridge
US5766550A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 14, 1996 |
| Grant date | Jun 16, 1998 |
| Priority date | — |
| Expiry date | Mar 14, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/1055
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A disposable cartridge for storage and delivery of reagents for synthesizing and labeling of synthetic oligonucleotides comprises an annular casing, an enclosed area defined by the interior surface of the casing and a non-swellable matrix which is inert to organic solvents is positioned within the enclosed area. Adsorbed onto the surface of the matrix is a reagent to be coupled to an immobilized oligonucleotide precursor. The cartridges can be packaged in such a way as to insure the long-term stability of the reagents contained therein. The cartridge is readily insertable into the reagent delivery line of an automated synthesizer of oligonucleotides and also is useful for the production of labeled oligonucleotides by manual coupling. The cartridges of the invention are disposable after a single use. Substantial economies in the synthesis of labeled synthetic oligonucleotides are achieved by use of the cartridge.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.