In vitro method for predicting the evolutionary response of a protein to a drug targeted thereagainst
US5766842A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 30, 1996 |
| Grant date | Jun 16, 1998 |
| Priority date | — |
| Expiry date | Aug 30, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/8142
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for identifying, in vitro, distinct, drug-resistant, biologically-active mutants of a protein that may emerge in vivo in response to a drug targeted against the protein is disclosed. The method involves preparing, by heterologous expression of a library of nucleotide sequences, a complete library of mutant proteins accessible in a single generation. The mutant proteins from the library that are drug resistant are identified. When all the first generation mutants have been identified in the above manner, a combination of drugs can be identified that will block the development of resistance. The same technique allows evaluation of the ultimate clinical efficacy of a drug targeted against the protein by comparing the number of resistant first generation mutants. A preferred protein is an HIV protease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.