Methods of amplifying nucleic acids using promoter-containing primer sequence
US5766849A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 28, 1994 |
| Grant date | Jun 16, 1998 |
| Priority date | — |
| Expiry date | Nov 28, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method, composition and kit for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH are provided in which multiple RNA copies of the target sequence autocatalytically generate additional copies using a mixture of blocked and unblocked primers and/or promoter-primers to initiate DNA and RNA synthesis, preferably with reduced non-specific product formation. The invention is useful for generating copies of a nucleic acid target sequence for purposes that include assays to quantitate specific nucleic acid sequences in clinical, environmental, forensic and similar samples, cloning and generating probes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.