Method for screening catalytic non-enzyme polypeptides and proteins
US5766861A · kind A · utility
0Cited by
0References
14Claims
0Family size
Assignees
Inventors
Key dates
| Filing date | Aug 22, 1995 |
| Grant date | Jun 16, 1998 |
| Priority date | — |
| Expiry date | Aug 22, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/901
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for screening or detection of a non-enzyme catalytic polypeptide or protein for the conversion of a substrate S to a product P is provided, in which a preparation containing the potential catalyst is contacted with the substrate S immobilized to a support and the immobilized product P obtained is detected, preferably by immunoassay. The method is preferably used for the screening of hybridoma supernatants for catalytic monoclonal antibodies that catalyze acyl transfer reactions, e.g., hydrolysis or aminolysis, condensation reactions and resolution of enantiomers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.