Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
US5766871A · kind A · utility
2Cited by
4References
15Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 27, 1996 |
| Grant date | Jun 16, 1998 |
| Priority date | — |
| Expiry date | Nov 27, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/98
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of screening for a given enzyme in colony-forming cells using a substrate of the enzyme that has low solubility. Use of such a method leads to the discovery of glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase of Pseudomonas nitroreducens. Also disclosed is a method of obtaining GL-7-ACA acylase from Pseudomonas cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.