Induction-free process for the recombinant preparation of glutarylamidase

US5766881A · kind A · utility

Assignee

• Hoechst GmbH · DE

Inventors

• Werner Aretz · Königstein, DE
• Ulrich Holst · Altenholz, DE
• Klaus-Peter Koller · Bad Soden, DE

Key dates

• Filing date: Oct 17, 1995
• Grant date: Jun 16, 1998
• Expiry date: Oct 17, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/72
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Improved, induction-free process for the recombinant preparation of glutarylamidase. The invention relates to a recombinant process for preparing glutarylamidase (GA), wherein the GA gene is expressed constitutively from an expression vector. In certain embodiments, the promoter for constitutively expressing the GA gene is a trc promoter or a trc-equivalent promoter which does not have any repressor or has a non-functional repressor.