Targeted nucleic acid delivery into liver cells
US5766899A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 27, 1995 |
| Grant date | Jun 16, 1998 |
| Priority date | — |
| Expiry date | Feb 27, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a receptor-mediated complex that selectively delivers nucleic acid into hepatocytes. Circumsporozoite (CS) protein is the targeting ligand that recognizes a receptor expressed on the liver cell surface. The CS ligand is complexed with a polylysine component that can bind nucleic acid. The level of gene expression is greatly enhanced when the complex is cotransfected with adenovirus. Using the present invention, a reporter gene was successfully transferred into a number of different cell lines that express high levels of receptor. The ability to introduce nucleic acid into specific mammalian cells is an important therapy for numerous diseases such as cancer, malaria and hepatitis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.