Method of regenerating fertile transgenic Zea mays plants from protoplasts

US5766900A · kind A · utility

Assignee

• Novartis AG · CH

Inventors

• Ray Shillito · Chapel Hill, US
• Gleta Carswell · Morrisville, US
• Christian Harms · Bad Krozingen, DE
• Yin-Fu Chang · Carrboro, US

Key dates

• Filing date: Apr 7, 1995
• Grant date: Jun 16, 1998
• Expiry date: Apr 7, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S47/01
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods of regenerating fertile Zea mays plants from protoplasts or protoplast-derived cells are described. The protoplasts or cells may be derived from embryogenic cell cultures or callus cultures. The protoplasts, cells and resulting plants may be transgenic, containing, for example, chimeric genes coding for a polypeptide having substantially the insect toxicity properties of the crystal protein produced by Bacillus thuringiensis.