Elimination of false negatives in nucleic acid detection
US5770360A · kind A · utility
4Cited by
6References
9Claims
0Family size
Assignee
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Key dates
| Filing date | May 24, 1995 |
| Grant date | Jun 23, 1998 |
| Priority date | — |
| Expiry date | May 24, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for the elimination of false negative test results in assays for the detection of amplified analyte nucleic acid in a sample. Prior to amplification, an internal control is added to the sample, comprising a nucleic acid distinguishable from the analyte nucleic acid, that can be amplified with the same amplification reagents as the analyte nucleic acid. Preferably the internal control comprises a nucleic acid sequence corresponding to the analyte nucleic acid that has been mutated to discriminate it from the analyte nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.