Tag reagent and assay method
US5770367A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 5, 1996 |
| Grant date | Jun 23, 1998 |
| Priority date | — |
| Expiry date | Feb 5, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/912
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A reagent comprises: a) an analyte moiety comprising at least two analyte residues, and linked to; b) a tag moiety comprising one or more reporter groups adapted for detection by mass spectrometry, wherein a reporter group designates analyte residue, and the reporter group at each position of the tag moiety is chosen to designate an analyte residue at a defined position of the analyte moiety. A plurality of such reagents, each comprising a different analyte moiety, provides a library of reagents which may be used in assay methods involving a target substance. Analysis of the tag moieties indicates the nature of the analyte moieties bound to the target substance. A method of sequencing nucleic acid employs a library of the reagents to determine the sequence of a target nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.