System and method for performing nucleic acid separations using liquid chromatography
US5772889A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 13, 1996 |
| Grant date | Jun 30, 1998 |
| Priority date | — |
| Expiry date | Nov 13, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2030/528
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Improved liquid chromatography systems having components made of titanium, coated stainless steel, or organic polymeric material are useful in the separation of nucleic acid fragments, particularly large fragments of double-stranded nucleic acids, by ion pairing reverse phase chromatography. The titanium, coated stainless steel, or polymeric components do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column may be employed to remove multivalent ions from the system. The multivalent cation capture resin may be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of nucleic acid fragments into fractions based on their molecular weight by ion pairing reverse phase chromatography and slalom chromatography utilize the liquid chromatographic systems described above.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.