Chemiluminescent reactions using dihydroxyaromatic compounds and heterocyclic enol phosphates
US5772926A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 13, 1997 |
| Grant date | Jun 30, 1998 |
| Priority date | — |
| Expiry date | May 13, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2334/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Novel methods and compositions which generate chemiluminescence are provided. The compositions comprise a heterocyclic enol phosphate compound and a dihydroxyaromatic compound in which the two hydroxy groups are separated by an even number of ring carbon atoms. Novel methods and compositions for generating chemiluminescence by reaction with a hydrolytic enzyme are provided as well. The compositions comprise a heterocyclic enol phosphate compound and a protected dihydroxyaromatic compound in which one of the hydroxy groups of the dihydroxyaromatic compound is protected with an enzyme-cleavable group. The novel chemiluminescent compositions are useful in methods for producing chemiluminescence for use in assays of hydrolytic enzymes and enzyme inhibitors and in assays employing labeled specific binding pairs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.