Photoactivatable o-nitrobenzyl polyethylene glycol-silane for the production of patterned biomolecular arrays
US5773308A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 10, 1997 |
| Grant date | Jun 30, 1998 |
| Priority date | — |
| Expiry date | Feb 10, 2017 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
O-nitrobenzyl analogs that include a photoremovable protecting group that resists the nonspecific adsorption of biomolecules and a linking group for attaching the o-nitrobenzyl analog to a substrate are disclosed. Also disclosed are methods for using o-nitrobenzyl analogs for creating patterned arrays of anti-ligands on a substrate so that a plurality of bioassays can be conducted simultaneously. In particular, the compounds disclosed are o-nitrobenzyl-polyethylene glycol-silanes, wherein the silane group serves to attach the compound to a substrate, the polyethylene glycol group resists the nonspecific adsorption of biomolecules. The o-nitrobenzyl group provides a photoactivatable functionality that allows the polyethylene glycol group to be selectively removed upon exposure to UV radiation and be replaced by an anti-ligand.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.