Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5773695A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 26, 1996 |
| Grant date | Jun 30, 1998 |
| Priority date | — |
| Expiry date | Jan 26, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/822
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A nuclear scaffold attachment region isolated from a tobacco gene, and a method of making recombinant cells having increased levels of expression of foreign genes therein, are described. The method comprises transforming the cell with a DNA construct comprising in the 5' to 3' direction, a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, and a scaffold attachment region of the nucleotide sequence provided herein, positioned either 5' to the transcription initiation region or 3' to the structural gene. DNA constructs and vectors employed in carrying out the foregoing method are also discussed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.