Method of enzyme immobilization on a particulate silica carrier for synthesis inorganic media
US5776741A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 19, 1996 |
| Grant date | Jul 7, 1998 |
| Priority date | — |
| Expiry date | Jul 19, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An immobilized enzyme is prepared for synthesis in a mainly organic medium devoid of free water. In one embodiment, an enzyme and liquid binder are introduced by atomization onto a particulate silica carrier having a particle size below 100 .mu.m in a granulator and simultaneously granulating to form the immobilized enzyme. In another embodiment, a liquid enzyme composition is contacted with the particulate silica carrier to obtain a particulate immobilized enzyme having a particle size below 100 .mu.m, the immobilized enzyme is introduced into granulator, a liquid binder is introduced into the granulator and granulation is carried out. The enzyme is preferably a lipase such as a thermostable lipase, and the immobilized lipase is used for interesterification of fats or synthesis of fatty acid esters.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.