Gene amplification methods
US5776746A · kind A · utility
18Cited by
6References
98Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | May 1, 1996 |
| Grant date | Jul 7, 1998 |
| Priority date | — |
| Expiry date | May 1, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/44
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention provides improved methods for the amplification and expression of recombinant genes in cells. The methods of the present invention permit the isolation of cell lines which have co-amplified input recombinant sequences which encode an amplifiable marker, one or more expression vectors encoding a protein of interest and optionally a selectable marker. The present methods allow the efficient isolation of amplified cell lines which express the protein(s) of interest in a relatively short period of time. The present invention also provides compositions comprising amplified T lymphoid cell lines.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.