Method for producing secretable glycosyltransferases and other golgi processing enzymes

US5776772A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• James G. Paulson · West Hollywood, US
• Eryn Ujita-Lee · Redondo Beach, US
• Karen J. Colley · Los Angeles, US
• Beverly S. Adler · Newbury Park, US
• Jeffrey K. Browne · Camarillo, US
• Jasminder Weinstein · Culver City, US

Key dates

• Filing date: Jan 30, 1996
• Grant date: Jul 7, 1998
• Expiry date: Jan 30, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/26
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferase.