Luminescence-scanning microscopy process and a luminescence scanning microscope utilizing picosecond or greater pulse lasers
US5777732A · kind A · utility
Inventors
Key dates
| Filing date | Apr 25, 1996 |
| Grant date | Jul 7, 1998 |
| Priority date | — |
| Expiry date | Apr 25, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/16
- WIPO fieldOptics
- WIPO sectorInstruments
Abstract
The description relates to a process for luminescence scanning microscopy with two-photon excitation, especially for examining biological objects (5). A laser pulse excites luminescent, especially fluorescing molecules and the luminescence emitted by the object (5) is measured and evaluated. In the process, the luminescent molecules in the object (5) are excited by laser pulses of over 10.sup.-12 second duration. A luminescence scanning microscope for implementing the process has a detector (2), a filter (7, 8) for separating the light emitted by the sample from the laser light (4) and a laser light source which is a laser (1) emitting pulsed or continuous radiation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.