Oligonucleotide probe conjugated to a purified hydrophilic alkaline phosphatase and uses thereof
US5780227A · kind A · utility
31Cited by
4References
23Claims
0Family size
Inventors
Key dates
| Filing date | Jun 7, 1995 |
| Grant date | Jul 14, 1998 |
| Priority date | — |
| Expiry date | Jun 7, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/916
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of preparing a homogeneous alkaline phosphatase-oligonucleotide probe conjugate having high specific enzyme activity for use in nucleic acid hybridization assays is disclosed. Indirect, competitive nucleic acid hybridization assay formats are also described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.