DNA sequencing, mapping, and diagnostic processes using hybridization and stable isotope labels of DNA
US5780232A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 28, 1996 |
| Grant date | Jul 14, 1998 |
| Priority date | — |
| Expiry date | May 28, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A DNA sequencing, mapping, and diagnostic process which includes the steps of labeling nucleotide segments or peptide nucleic acids (PNAs) with one or more atoms of specific stable or long-lived radioactive isotopes of a selected element that do not normally occur in DNAs, ODNs or PNAs such that the nucleotide segment or PNA has specific stable or long-lived radioactive isotope of a specified selected element at a terminal or an interior position; hybridizing the labeled nucleotide segment or PNA to complementary nucleic acid segments or PNAs which are fixed on a hybridization surface; and, using mass spectrometric techniques, including RIS, to analyze the presence and position of the labeled hybridized nucleotide segments or PNAs which are bound to the fixed nucleotide segments or PNAs.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.