Artificial mismatch hybridization

US5780233A · kind A · utility

Assignee

• Wisconsin Alumni Research Foundation · US

Inventors

• Zhen Guo · Cheltenham, US
• Lloyd M. Smith · Atlanta, US

Key dates

• Filing date: Jun 6, 1996
• Grant date: Jul 14, 1998
• Expiry date: Jun 6, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6827
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

An improved nucleic acid hybridization process is provided which employs a modified oligonucleotide and improves the ability to discriminate a control nucleic acid target from a variant nucleic acid target containing a sequence variation. The modified probe contains at least one artificial mismatch relative to the control nucleic acid target in addition to any mismatch(es) arising from the sequence variation. The invention has direct and advantageous application to numerous existing hybridization methods, including, applications that employ, for example, the Polymerase Chain Reaction, allele-specific nucleic acid sequencing methods, and diagnostic hybridization methods.