Patent · US Expired

Immobilization of penicillin G amidase, glutaryl-7-ACA acylase or D-aminoacid oxidase on an aminofunctional organosiloxane polymer carrier

US5780260A · kind A · utility

3Cited by

0References

23Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Frank Wedekind · Penzberg, DE
Adelheid Daser · Schlehdorf, DE
Wilhelm Tischer · Peißenberg, DE

Key dates

Filing date	May 30, 1996
Grant date	Jul 14, 1998
Priority date	—
Expiry date	May 30, 2016

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N11/14
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

An enzyme selected from penicillin-G amidase, glutaryl-7-ACA acylase and D-amino acid oxidase is immobilized by covalent bonding on an aminofunctional organosiloxane polymer carrier to provide an immobilized enzyme having a specific volume activity of at least 100 U/g wet carrier. Preferably, the carrier has an average diameter or 0.01 to 3 mm and is essentially spherical. Covalent bonding is accomplished by activating amino groups on the carrier with a dialdehyde and reacting the activated groups with reactive groups on the enzyme. An amount of enzyme is immobilized to provide a weight ratio of enzyme to carrier of 1 to 300 mg protein per g wet carrier.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.