"Method for purification of protein ""e"" from haemophilus influenzae"
US5780601A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 23, 1995 |
| Grant date | Jul 14, 1998 |
| Priority date | — |
| Expiry date | May 23, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/40
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
"A method of purifying protein "e" from Haemophilus influenzae includes disrupting H. influenzae cells, subjecting the disrupted cells by differential sedimentation to obtain a total cell membrane fraction, fractionating the total cell membrane into inner and outer membrane components by density gradient sedimentation or by differential solubilization of the inner membrane component with detergents, obtaining a subfraction of the preparation of the outer membrane components which is enriched in protein "e" by extraction with an aqueous solution of 0.1-2.0% N-lauroyl sarcosine, sodium salt, solubilizing the protein "e" from the subfraction by a two-step differential solubilization process with sulfobetaine detergents, and recovering the aqueous solution which contains the purified protein "e".
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.