Enzymatic oxidation of alcohols to aldehydes in a continuous reaction system
US5783429A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 9, 1996 |
| Grant date | Jul 21, 1998 |
| Priority date | — |
| Expiry date | Oct 9, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/921
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the enzymatic oxidation of alcohols to aldehydes in a continuous reaction series is disclosed utilizing crude cellular methanol oxidase and catalase produced by an acceptable organism such as those selected from the genera of Pichia, Hansenula, Candida and Torulopsis. The process maintains a steady-state aldehyde concentration whereby enzyme activity is promoted. In particular, the process has two reactors operating in series continuously producing a fermenter effluent containing growing, intact yeast cells at a cell concentration of about 0.5 to about 3% by weight of the effluent by continuous culture of the cells on a methanol carbon source, continuously introducing the effluent from the first reactor to the second reactor, adding an alcohol feed at about 0.2-10% w/w and oxygen to the second reactor, maintaining a steady-state aldehyde concentration in the reaction mixture, the concentration of residual alcohol being about 0.1-9% w/w by controlling the rates of introduction of effluent and alcohol feed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.