Non-separation specific binding chemiluminescent assay

US5783453A · kind A · utility

Assignee

• Chiron Diagnostics Corporation · US

Inventors

• Eve H. Barlow · Princeton, US
• Eddie Carroll · Norwood, US
• Joseph E. Connolly · Dedham, US
• Michael Lee · Orlando, US
• Richard A. Martinelli · Watertown, US
• John T. Unger · Medfield, US

Key dates

• Filing date: Jun 29, 1995
• Grant date: Jul 21, 1998
• Expiry date: Jun 29, 2015

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/582
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The assay described herein is predicated on an observation that when acridinium ester labeled tracers are bound to their corresponding binding conjugate immobilized on a metal oxide solid phase, the measurable chemiluminescent light emission of the labeled tracer bound to the solid phase is quenched as compared to the free fraction tracer that is unattached to the solid phase. According to the invention, a non-separation specific binding assay to detect or quantify the presence of an analyte is provided where the entire reaction mixture is flashed (including unreacted tracer) and modulated signal (because of the quench effect) is associated with a reference, thus determining the amount or presence of said analyte in said sample. Disadvantages inherent in heterogeneous assays employing multiple separations may be avoided using this non-separation method.