Androgen regulation with DNA sequences of rat probasin gene
US5783681A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Mar 8, 1995 |
| Grant date | Jul 21, 1998 |
| Priority date | — |
| Expiry date | Mar 8, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/008
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A DNA sequence (-426 to +28 base pairs) cloned from the probasin (PB) gene promoter region confers androgen regulation in cell culture and prostate specific expression in transgenic non-human eukaryotic animals. Various PB promoter fragments impart preferential regulation by androgens compared to other steroid hormones on fused transgenes. Alteration of the DNA sequences and/or combinations permits production of an (1) assay for androgenic or anti-androgenic materials, (2) transgenic non-human eukaryotic animal models for prostatic disease, (3) cell culture models for prostatic disease, and (4) treatment of human benign prostatic hyperplasia and human prostate cancer by gene therapy. This invention permits assays on agonist and antagonist of the androgen receptor or pathways that result in androgen action, testing materials for carcinogenicity of the prostate, testing drugs and gene therapy, or protection potential of materials on prostatic cells against prostatic disease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.