Chemical preparation of high specific activity nucleotide probes
US5783685A · kind A · utility
1Cited by
16References
7Claims
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Key dates
| Filing date | Mar 21, 1996 |
| Grant date | Jul 21, 1998 |
| Priority date | — |
| Expiry date | Mar 21, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Methods of chemical synthesis of labeled nucleotide probes are described. The methods comprise providing a first target recognition moiety comprising a nucleotide sequence of at least about 15 nucleotide bases including at least one 5' end and 3' end. The first target recognition moiety is chemically altered to contain a carboxyl group. A second signal generating moiety comprising a hydroxyl group is provided. The carboxyl group of the first target recognition moiety is chemically reacted with hydroxyl group of the second signal generating moiety to chemically join the two moieties and produce said labeled nucleotide probe.
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