Dyes designed for high sensitivity detection of double-stranded DNA
US5783687A · kind A · utility
30Cited by
3References
2Claims
0Family size
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Key dates
| Filing date | Sep 5, 1996 |
| Grant date | Jul 21, 1998 |
| Priority date | — |
| Expiry date | Sep 5, 2016 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.