Method and kit for detecting nucleic acid cleavage utilizing a covalently attached fluorescent tag
US5786139A · kind A · utility
43Cited by
4References
16Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 9, 1994 |
| Grant date | Jul 28, 1998 |
| Priority date | — |
| Expiry date | Dec 9, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6823
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is based on the degradation of a fluorescence labeled nucleic acid such as DNA (F-DNA), RNA (F-RNA) or DNA/RNA substrate. The label may be covalently attached to the relatively high volume substrate at one position or many positions. The nucleic acid is then put in contact with another substance and activity is detected by Fluorescence Polarization.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.