Electrogenerated chemiluminescence labels for analysis and/or referencing
US5786141A · kind A · utility
Inventors
Key dates
| Filing date | Feb 9, 1995 |
| Grant date | Jul 28, 1998 |
| Priority date | — |
| Expiry date | Feb 9, 2015 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/582
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Biomolecule analysis using anodic oxidation of aqueous sodium 9, 10-diphenylanthracene-2-sulfonate (DPAS) and 1- and 2-thianthrenecarboxylic acid (1-THCOOH and 2-THCOOH) in the presence of tri-n-propylamine (TPrA) as a coreactant in aqueous solution produces electrogenerated chemiluminescence (ECL). In addition, the cathodic reduction of DPAS in the presence of peroxydisulfate (S.sub.2 O.sub.8.sup.2-) as a coreactant also produces ECL in an acetonitrile (MeCN)-water solution (1:1 by volume). The oxidation of chlorpromazine (CPZ) produces an ECL emission in the absence of an added coreactant following an unprecedented "self-annihilation" mechanism.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.