Method for screening an expression cDNA clone bank for the detection of polynucleotides

US5786466A · kind A · utility

Assignee

• Biomay Produktions-Und Handelsgesellschaft m.b.H. · AT

Inventors

• Michael Breitenbach · Wien, AT
• Dietrich Kraft · Wien, AT
• Helmut Rumpold · Wien, AT
• Otto Scheiner · Perchtoldsdorf, AT
• Heimo Breiteneder · Wien, AT
• Karin Pettenburger · Wien, AT
• Rudolf Valenta · Theresienfeld, AT

Key dates

• Filing date: Jun 6, 1995
• Grant date: Jul 28, 1998
• Expiry date: Jun 6, 2015

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/5308
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a method by which expression cDNA clone banks are screened by means of IgE antibodies derived from the sera of allergic individuals, or by means of monoclonal or polyconal antibodies. This type of screening is used for the detection of polynucleotides whose open-reading frames code for allergenically active proteins. These proteins are characterized in that their biological activity as allergens is equal to that of plant allergens occurring in nature.