System for assaying binding to a heparin-binding growth factor receptor

US5789182A · kind A · utility

Assignees

• Children's Medical Center Corporation · US
• President and Fellows of Harvard College · US

Inventors

• Avner Yayon · Afula, IL
• David M. Ornitz · Creve Coeur, US
• Michael Klagsbrun · Newton, US
• Philip Leder · Brookline, US
• John Flanagan · Berkeley, US

Key dates

• Filing date: Dec 14, 1993
• Grant date: Aug 4, 1998
• Expiry date: Dec 14, 2013

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A homogeneous population of cells having on average (1) a number of cell surface low-affinity heparin-binding growth factor (HBGF) sites per cell less than 20% of the number of such binding sites found on wild-type CHO-K1 cells (ATCC Accession No. CCL61), and at least three times the number of cell surface high-affinity HBGF receptors per cell found on such CHO-K1 cells; and an assay system utilizing such cells.