Expression in non-tumoral human lymphoblastoid lines with an integrative vector
US5789247A · kind A · utility
Inventors
Key dates
| Filing date | Oct 12, 1994 |
| Grant date | Aug 4, 1998 |
| Priority date | — |
| Expiry date | Oct 12, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A plasmid vector designed for transforming lymphoblastoid cells, immortalized by Epstein Barr virus, for producing heterologous proteins, which comprises the bacterial DNA elements required for its amplification together with the following DNA fragments: PA1 the DNA elements which constitute an expression cassette allowing the insertion of a heterologous DNA to be expressed in the human lymphoblastoid cells, PA1 the Adenovirus 5 derived DNA sequence encoding the VA I and VA II RNA aimed at optimizing the messenger RNA translation, PA1 a gene engineered to be efficient as a selective marker in eukaryotic cells, and PA1 DNA segments selected for their property to promote plasmid DNA integration into the cell chromosome DNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.