Transgenic animals expressing artificial epitope-tagged proteins

US5789655A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Stanley B. Prusiner · San Francisco, US
• Glenn C. Telling · San Francisco, US
• Fred E. Cohen · Los Angeles, US
• Michael R. Scott · Kama'oa Homesteads, US

Key dates

• Filing date: Jun 6, 1996
• Grant date: Aug 4, 1998
• Expiry date: Jun 6, 2016

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/2828
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

DNA constructs are provided of epitope-tagged proteins or protein fragments which are conveniently purified with immunoaffinity chromatography such as epitope-tagged prion proteins (PrP). Transgenic animals expressing an epitope-tagged protein are provided, including transgenic animals expressing epitope-tagged PrP. Methods for distinguishing between the conformational shapes of a protein and a convenient method for isolating a tagged protein by immunoaffinity chromatographic methods are provided.