Method of identifying MHC-class I restricted antigens endogenously processed by cellular secretory pathway
US5792604A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 12, 1996 |
| Grant date | Aug 11, 1998 |
| Priority date | — |
| Expiry date | Mar 12, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of identifying antigens which are capable of being endogenously processed by a cellular secretory pathway comprising: introducing an antigen into a donor cell lacking in MHC class I molecules, incubating in an in vitro medium the donor cells, primed cytotoxic T lymphocytes having specificity for the antigen, and target cells which express MHC class I molecules and are labelled with a detectable intracellular marker, under suitable conditions such that the donor cells remain intact, and measuring the amount of detectable marker released into the incubation medium. A method of assaying a medium for the presence of a substance that affects processing of an endogenously processed antigen in a cellular secretory pathway, and; a method of characterizing a tumor or viral antigen capable of being endogenously processed by a cellular secretory pathway.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.