Methods for preserving recombinant retroviruses
US5792643A · kind A · utility
Inventors
Key dates
| Filing date | Mar 28, 1997 |
| Grant date | Aug 11, 1998 |
| Priority date | — |
| Expiry date | Mar 28, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/10051
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Methods for preserving an infectious recombinant virus for subsequent reconstitution are provided. Within one aspect, the method comprises the steps of (a) combining an infectious recombinant virus with an aqueous solution comprising a saccharide, a high molecular weight structural additive, a buffering component and water to form an aqueous suspension, thereby stabilizing the infectious virus; (b) cooling the aqueous suspension containing the virus to a temperature below the glass transition state temperature or below the eutectic point temperature of the formulation; and (c) removing water from the cooled aqueous suspension by sublimation to form a lyophilized virus having less than 10% water by weight of the lyophilized virus, the virus being capable of infecting mammalian cells upon reconstitution.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.