Enhancement of the transfection efficiency of nucleic acids by using isopropanol in aqueous solutions

US5792651A · kind A · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Metin Colpan · Essen, DE
• Joachim Schorr · Düsseldorf, DE

Key dates

• Filing date: Oct 11, 1996
• Grant date: Aug 11, 1998
• Expiry date: Oct 11, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/101
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Use of isopropanol in aqueous solutions for chromatographic isolation of nucleic acids for enhancing the transfection efficiency of the isolated nucleic acids in prokaryotic and eukaryotic cells.