Detecting prions in a sample and prion preparation and transgenic animal used for same

US5792901A · kind A · utility

Assignee

• The Regents of the University of California · US

Inventors

• Stanley B. Prusiner · San Francisco, US
• Michael R. Scott · Kama'oa Homesteads, US
• Glenn C. Telling · San Francisco, US

Key dates

• Filing date: Jul 30, 1996
• Grant date: Aug 11, 1998
• Expiry date: Jul 30, 2016

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/2828
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention includes an artificial PrP gene, a transgenic animal containing a PrP gene of another animal or the artificial PrP gene, a hybrid non-human mammal with an ablated endogenous prion protein gene and exogenous prion protein gene, assay methodology which uses the animals to detect pathogenic prions in a sample and standardized prion preparation used in the assay. The genome of a host animal (such as a mouse), is manipulated so that the animal is rendered susceptible to infection with prions which normally would infect only a genetically diverse test animal (such as human, cow or sheep). A PrP gene of the host is preferably manipulated to include a mutation which matches a mutation which causes prion disease in the genetically diverse mammal. Pathogenic prions in a sample can be detected by injecting the sample to be tested into a mammal of the invention which has been genetically manipulated so as to be susceptible to infection from prions in the sample. Mammals which are not inoculated with the sample and others inoculated with a standardized prion preparation of the invention are used as controls in the assay to detect prions in samples which cause diseases. For example…