Process and device for the separation and detection of components of a mixture of materials by temperature gradient gel electrophoresis

US5795720A · kind A · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Karsten Henco · Erkrath, DE
• Detlev Riesner · Düsseldorf, DE
• Gerhard Steger · Düsseldorf, DE

Key dates

• Filing date: Oct 31, 1995
• Grant date: Aug 18, 1998
• Expiry date: Oct 31, 2015

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/44704
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

The invention relates to a process for the separation and detection of components of a mixture of materials by temperature gradient gel electrophoresis, wherein either PA1 a spatial temperature gradient is built up by spatially separated temperature levels, or PA1 a time temperature gradient, or PA1 a temperature gradient is built up by combination of spatial and timewise temperature gradient. The temperature levels for building up the spatial temperature gradient are adjusted by controllable heating or cooling devices. To build up the time temperature gradient, the temperature level at each point of the separation path within the separation medium may be optionally adjusted time-dependently by means of controllable heating or cooling devices. There is described a device for performing the process with controllable heating or cooling devices to build up temperature gradients, a hollow body arranged between the temperature levels which contains the medium used for separation, and a thermostat jacket enclosing the hollow body.