Process for manufacturing new biopolymers

US5795747A · kind A · utility

Assignee

• EVOTEC BIOSYSTEMS GMBH · DE

Inventors

• Karsten Henco · Erkrath, DE
• Manfred Eigen · Göttingen, DE

Key dates

• Filing date: Jun 26, 1995
• Grant date: Aug 18, 1998
• Expiry date: Jun 26, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6811
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The process of generating new biopolymers with improved characteristics, assisted by polymerases, is characterized in that at least one cycle of the steps described below, will be performed in a series of parallel preparations which are to be compared. Sequences of nucleic acids or, a mixture of similar sequences of nucleic acids in the distribution of mutants of a quasi-species, are subjected, in the region of the error threshold, to limited mutagenesis. These mixtures are replicated under simultaneous conditions and/or in succession. The mixtures of the so product nucleic acids are compartmentalized by segregation, and thereafter selected by a selection system which reflects the characteristics of interest of the nucleic acid sequence itself or, indirectly, via its translation product.