Method for creating superinduced cDNA library & isolating ligand-stimulated genes
US5795752A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Oct 27, 1994 |
| Grant date | Aug 18, 1998 |
| Priority date | — |
| Expiry date | Oct 27, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1096
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method of producing a complementary DNA (cDNA) library enriched in ligand-inducible genes of a cell. The method includes activating a cellular ligand receptor for a predetermined period of time in the presence of labelled RNA precursors and a substance which enhances the level of RNA in said cell. The RNA presursors are incorporated into the RNA synthesized by said cell in response to receptor activation. The labelled RNA is separated from unlabelled RNA and used to prepare cDNA. The cDNA is cloned into host cells to provide a cDNA library of cDNA-containing clones. This library can then be screened for clones containing ligand-inducible genes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.