Mutants of 2,5-diketo-D-gluconic acid (2,5-DKG) reductase A
US5795761A · kind A · utility
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17References
12Claims
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Key dates
| Filing date | Jan 16, 1996 |
| Grant date | Aug 18, 1998 |
| Priority date | — |
| Expiry date | Jan 16, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/843
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Mutants of 2,5-diketo-D-gluconic acid reductase A an enzyme used to produce 2-keto-L-gluconic acid, a precursor of ascorbic acid (vitamin C), are prepared by site-directed mutagenesis. These mutants may exhibit one or more of the following characteristics: improved temperature stability, increased resistance to substrate inhibition, increased turnover of the substrate by the enzyme and increased affinity for the substrate.
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