Patent · US Expired

Device for detecting microorganisms

US5795773A · kind A · utility

52Cited by
20References
29Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMar 24, 1997
Grant dateAug 18, 1998
Priority date
Expiry dateMar 24, 2017

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/04
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A method and a sealable, sterilizable vessel for detecting the presence of microorganisms in a specimen, the vessel containing a liquid culture medium and a sensor with an indicator medium therein. A gas permeable membrane with a gas impermeable removable seal thereon are provided in a wall of the vessel. If the microorganism to be detected is an anaerobic organism, then the gas impermeable seal is left in place. If the microorganism to be detected is an aerobic organism, then the gas impermeable seal is removed so as to allow the passage of oxygen into the vessel. In a preferred embodiment, the gas permeable membrane allows the passage of oxygen into the vessel while at least partly restricting the passage of carbon dioxide to outside of the vessel. Changes in the indicator medium resulting from a change in gas production (CO.sub.2, NH.sub.2, H.sub.2 S, etc.), volatile acid production or pH concentration in the medium, are detected from outside the vessel.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.