Method for controlling proliferation and differentiation of cells encapsulated within bioartificial organs

US5795790A · kind A · utility

Assignee

• Cytotherapeutics, Inc. · US

Inventors

• Malcolm Schinstine · Lehighton, US
• Molly S. Shoichet · Toronto, CA
• Frank T. Gentile · East Greenwich, US
• Joseph P. Hammang · Barrington, US
• Laura M. Holland · Ambler, US
• Brian M. Cain · Everett, US
• Edward J. Doherty · Halifax, US
• Shelley R. Winn · Providence, US
• Patrick Aebischer · Lutry, CH

Key dates

• Filing date: May 23, 1995
• Grant date: Aug 18, 1998
• Expiry date: May 23, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/74
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods and compositions are provided for controlling cell distribution within a bioartificial organ by exposing the cells to a treatment that inhibits cell proliferation, promotes cell differentiation, or affects cell attachment to a growth surface within the bioartificial organ. Such treatments include (1) genetically manipulating cells, (2) exposing the cells to a proliferation-inhibiting compound or a differentiation-inducing compound or removing the cells from exposure to a proliferation-stimulating compound or a differentiation-inhibiting compound; exposing the cells to irradiation, and (3) modifying a growth surface of the bioartificial organ with extracellular matrix molecules, molecules affecting cell proliferation or adhesion, or an inert scaffold, or a combination thereof. These treatments may be used in combination. In a preferred treatment, cells are exposed to and then removed from exposure to a proliferation-stimulating and differentiation inhibiting compound prior to encapsulation of the cells in a semipermeable biocompatible jacket to form a bioartificial organ. Upon in vivo implantation of the bioartificial organ in a host, cellular proliferation is inhibited and …