Methods for screening of test compounds for inhibiting binding of a CD4-HIV 1 complex to a chemokine receptor
US5798206A · kind A · utility
12Cited by
2References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 10, 1997 |
| Grant date | Aug 25, 1998 |
| Priority date | — |
| Expiry date | Jan 10, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/56988
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for the screening of a test compound for inhibiting the binding of a CD4-HIV 1 complex to HIV-1 second receptors, comprising: (a) preparing a magnetic ligand by mixing a magnetic, CD4-containing substrate with HIV-1, (b) mixing the magnetic ligand from step (a) with a test compound, (c) adding cells that express the HIV-1 second receptors to the mixture from step (b), (d) separating cells with bound magnetic ligands from cells without bound magnetic ligands by contact with a magnetic separator, and (e) quantifying the cells with bound magnetic ligands and quantifying the cells without bound magnetic ligands.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.