Method of analyzing tumor cell DNA content through tumor cell enrichment
US5798217A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 12, 1995 |
| Grant date | Aug 25, 1998 |
| Priority date | — |
| Expiry date | Jun 12, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25125
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a method for enriching a sample in tumor cells and determining the DNA ploidy and proliferative index of the tumor cells present in the sample. The method uses a pan-leukocyte monoclonal antibody conjugated to a separable substrate such as magnetic microspheres to conjugate and remove leukocytes from the sample. A fluorescently labelled monoclonal antibody specific to a tumor associated antigen or an antigen arising from the presence of the tumor and a DNA staining reagent are added to the leukocyte depleted sample. The sample is then analyzed by dual color flow cytometry to determine cells having an abnormal DNA content by gating on fluorescently labelled cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.