Method for the directed modification of enzymes, modified enzymes and their use
US5798234A · kind A · utility
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Key dates
| Filing date | Apr 1, 1997 |
| Grant date | Aug 25, 1998 |
| Priority date | — |
| Expiry date | Apr 1, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P13/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Enzymes with modified substrate specificity and methods of obtaining same by the steps of a) determining the structure of two or more enzymes in the same group, b) comparing the structure of the substrate binding sites of the enzymes, and c) modifying selected amino acids by genetic mutation so that the substrate preference of one of the enzymes is altered. The methods are particularly useful for the stereospecific interconversion of oxyacids and aminoacids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.