Method for chromosomes classification
US5798262A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 18, 1997 |
| Grant date | Aug 25, 1998 |
| Priority date | — |
| Expiry date | Apr 18, 2017 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6441
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for finding L internal reference vectors for classification of L chromosomes or portions of chromosomes of a cell, the L chromosomes or portions of chromosomes being painted with K different fluorophores or combinations thereof, wherein K basic chromosomes or portions of chromosomes of the L chromosomes or portions of chromosomes are each painted with only one of the K different fluorophores, whereas the other L-K of the L chromosomes or portions of chromosomes are each painted with a different combination of the K different fluorophores, the method comprising the steps of (a) using a multi-band collection device for measuring a first vector for each pixel of each of the L chromosomes or portions of chromosomes; (b) identifying pixels belonging to each of the K basic chromosomes or portions of chromosomes and defining the pixels as basic pixels, so as to obtain K basic classes of basic pixels; (c) using at least one basic pixel from each of the K basic classes for obtaining K basic vectors, the K basic vectors being K internal reference vectors; (d) using the K basic vectors for identifying pixels belonging to the other L-K chromosomes or portions of chromosomes; and (e) u…
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